4,179 research outputs found

    Studies on the cellular localisation of Poly ADP Ribose Polymerase (PARP)-14

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    The poly(ADP-ribose) polymerase (PARP) family consists of 17 proteins, some of which are involved in DNA repair, regulation of transcription and mediation of telomere length. PARP-14 is the largest molecule in this family and has already been shown to play a nuclear role in regulating Stat6-activated transcription, TH2 differentiation, and preventing apoptosis in B cells. This thesis describes work that has explored the hypothesis that PARP-14 also has a role in the cytoplasm. Based on observations that GFP-tagged full length PARP-14 and a GFP-tagged fragment of PARP-14 consisting of the region from the third macro domain (M3) to the WWE domain (M3-WWE) both localise to granules in the cytoplasm of Hela cells, we initially used M3-WWE as bait in a yeast two-hybrid screen to search potential binding partners. This failed to identify any definite and relevant hits for follow-up. Using PHYRE (a web-based protein fold recognition programme established at Imperial College), we subsequently discovered three previously unrecognized degenerate KH domains in PARP-14, with one (KH3) situated between the third macro domain and WWE domains. Using a panel of GFP-fusion proteins and confocal microscopy, we found that KH3-GFP localises to cytoplasmic granules in Hela cells in a similar manner to full length PARP-14, which in turn showed a similar distribution to that of antibody-detected endogenous PARP-14 in mouse macrophages. Using a panel of antibodies against protein components of stress granules, exosomes and lysosomes, we found that PARP-14 colocalises with stress granules in cells stressed by sodium arsenite, but with exosome complex proteins in cells activated by LPS. Co-immunoprecipitation experiments confirmed that KH3-GFP associates with eIF3η and TTP and that these associations were RNA-dependent manner. These observations support a role for PARP-14 in regulating RNA processing in stress granules and exosomes, and suggest that the KH3 domain is critical for PARP-14 cytoplasmic granule localisation.Open Acces

    Effective thermal management of multiple electronic components

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    [[abstract]]The objective of this paper is to provide an effective and accurate analytical solution to compute the spreading thermal resistance of a vapor chamber thermal module, as well as the surface temperatures and the heat flux distributions at the heating surface. The analytical solutions are expressed in a reduced unit system with the governing parameters of the corresponding distance between heat sources, dimensionless plate thickness of the vapor chamber. This paper also presents vapor chamber temperature distribution, and it is correlation to heat source sizes, hence, spreading thermal resistance decreases with the increasing lateral length. There is the obvious difference between spreading thermal and conductive thermal resistance as lateral length is disproportion to heating area. Therefore, spreading thermal resistance is an important factor when design the thermal solution of a high density chipset power, and it caused high temperature in heat sources by embedded a thinner heat sink base. According to Fourier conductivity theorem, spreading thermal resistance is disproportion to sink base, then thermal resistance is not only parameter for vapor chamber module design, it needs to consider spreading resistance of vapor chamber and fin performance for cooling LEDs array, in order to prevent mismatch on numerical analysis and mathematical calculation. Thermal simulation is used as a design tool, and it is close to experimental data. The difference is within 5.9%, and it presents a precise result.[[conferencetype]]國際[[conferencedate]]20101020~20101022[[iscallforpapers]]Y[[conferencelocation]]Taipei, Taiwa

    Review on the Conflicts between Offshore Wind Power and Fishery Rights: Marine Spatial Planning in Taiwan

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    In recent years, Taiwan has firmly committed itself to pursue the green energy transition and a nuclear-free homeland by 2025, with an increase in renewable energy from 5% in 2016 to 20% in 2025. Offshore wind power (OWP) has become a sustainable and scalable renewable energy source in Taiwan. Maritime Spatial Planning (MSP) is a fundamental tool to organize the use of the ocean space by different and often conflicting multi-users within ecologically sustainable boundaries in the marine environment. MSP is capable of definitively driving the use of offshore renewable energy. Lessons from Germany and the UK revealed that MSP was crucial to the development of OWP. This paper aims to evaluate how MSP is able to accommodate the exploitation of OWP in Taiwan and contribute to the achievement of marine policy by proposing a set of recommendations. It concludes that MSP is emerging as a solution to be considered by government institutions to optimize the multiple use of the ocean space, reduce conflicts and make use of the environmental and economic synergies generated by the joint deployment of OWP facilities and fishing or aquaculture activities for the conservation and protection of marine environments.Peer Reviewe

    Negative entanglement measure for bipartite separable mixed states

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    We define a negative entanglement measure for separable states which shows that how much entanglement one should compensate the unentangled state at least for changing it into an entangled state. For two-qubit systems and some special classes of states in higher-dimensional systems, the explicit formula and the lower bounds for the negative entanglement measure have been presented, and it always vanishes for bipartite separable pure states. The negative entanglement measure can be used as a useful quantity to describe the entanglement dynamics and the quantum phase transition. In the transverse Ising model, the first derivatives of negative entanglement measure diverge on approaching the critical value of the quantum phase transition, although these two-site reduced density matrices have no entanglement at all. In the 1D Bose-Hubbard model, the NEM as a function of t/Ut/U changes from zero to negative on approaching the critical point of quantum phase transition.Comment: 6 pages, 3 figure
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